• 论文
主办单位:煤炭科学研究总院有限公司、中国煤炭学会学术期刊工作委员会
神东矿区接种AMF对植物−土壤生态化学计量及养分回收的影响
  • Title

    Effects of AMF inoculation on plant-soil ecological stoichiometry and nutrient recovery in Shendong mine

  • 作者

    王董董肖礼毕银丽聂文杰张可

  • Author

    WANG Dongdong;XIAO Li;BI Yinli;NIE Wenjie;ZHANG Ke

  • 单位

    西安科技大学 西部矿山生态环境修复研究院中国矿业大学(北京)煤炭资源与安全开采国家重点实验室

  • Organization
    Institute of Ecological Environment Restoration in Mine Areas of West China, Xi’an University of Science and Technology
    State Key Laboratory of Coal Resources and Safe Mining, China University of Mining and Technology-Beijing
  • 摘要

    矿区采煤沉陷地植被恢复和微生物复垦是打造绿色矿山的关键点。丛枝菌根真菌( Arbuscular Mycorrhizal Fungi ,AMF)和恢复植被类型对植物−枯落叶−土壤连续体的生态化学计量学特征及养分回收的影响尚不明朗,但对阐明西北干旱采煤沉陷区生态修复后养分循环和可持续发展具有重要意义。选择复垦10 a后接种AMF与对照的紫穗槐、沙棘、文冠果、欧李、野樱桃5种恢复植被及对应植被的0~10 cm土壤进行研究,旨在探明接菌和恢复植被类型对植物−枯落叶−土壤连续体碳(C)、氮(N)、磷(P)含量和生态化学计量比,养分回收率、养分限制的影响。结果表明:5种恢复植被各器官平均养分含量与全国水平相比呈现出高C、P低N的特点,土壤N的缺乏限制了枯落物分解和植物生长。冗余分析表明影响植物生态化学计量的主要土壤因子是土壤有机碳、P、土壤易提取球囊霉素并且土壤养分与叶养分回收在菌根介导下呈正相关。AMF接种提高了植物、土壤养分含量、N、P回收率和土壤碳氮比,降低了植被的氮磷比,进而缓解植物N限制,增加植物生长速率支持恢复植被重建,促进连续体养分循环。在不同人工重建植被中接菌的固氮植被紫穗槐和沙棘养分含量、稳定性及生长速率高。植物−枯落叶−土壤连续体生态化学计量及养分回收特征预测土壤氮限制沉陷区可持续生态恢复,接种AMF则促进沉陷区持续生态恢复的潜力。

  • Abstract

    Vegetation restoration and microbial reclamation of mining subsidence area is the key spot to construct green mine.The effects of Arbuscular Mycorrhizal Fungi (AMF) and re-vegetation types on the ecological chemometric characteristics of the plant-litter-soil continuum and leaf nutrient resorption are still unclear, but are important for illuminating nutrient cycling and sustainable development after ecological restoration in arid coal mining subsidence areas in Northwest China. Five types of restored vegetation and the 0~10 cm soil of the corresponding vegetation were selected for study in the reclamation of 10 years of inoculation with AMF and the control area— Amorpha fruticosa , Hippophae rhamnoides , Xan-thoceras sorbifolium , Cerasus humilis and Cerasus szechuanica .The intention was to explore the influences of inoculum and re-vegetation type on C, N and P contents and ecological stoichiometric ratios, nutrient recovery rates, and nutrient limitation within the plant-litter-soil continuum. Compared with the national level, the average nutrient content of the five restored vegetation organs showed the characteristics of higher C, lower P and lower N. The decomposition of the litter and plant growth were limited by the deficiency of soil N.Redundancy analysis indicates that the main soil factors affecting plant ecological stoichiometry were soil organic carbon, P, easily extracta-ble-glomalin-related soil protein and positive correlation between soil nutrients and leaf nutrient resorption mediated by mycorrhizae.AMF inoculation enhanced plant and soil nutrient content, N and P recovery rates and C:N, reduced vegetation N:P, which in turn alleviated plant N limitation, increased plant growth rate supporting the restoration of revegetation and promoting nutrient cycling along the continuum.On the other hand, the nutrient content, stability and growth rate of the inoculated nitrogen-fixing vegeta-tion in different artificially reconstructed vegetation of Amorpha fruticose and Hippophae rhamnoides were high. Ecological stoichiometry and nutrient recovery characteristics of the plant-litter-soil continuum pre-dicted soil nitrogen-limited sustainable ecological restoration in subsidence areas, while inoculation with AMF promoted the potential of sustainable ecological restoration in subsidence areas.

  • 关键词

    采煤沉陷地丛枝菌根真菌矿区生态修复生态化学计量

  • KeyWords

    coal mining subsidence sites;Arbuscular Mycorrhizal fungi(AMF);ecological restoration;ecological stoichiometry

  • 基金项目(Foundation)
    国家自然科学基金青年项目资助项目(52204189);陕西省自然科学基础研究计划资助项目(2022JQ-436);陕西省教育厅科学研究计划(22JK0464);
  • DOI
  • 引用格式
    王董董,肖 礼,毕银丽,等. 神东矿区接种AMF对植物−土壤生态化学计量及养分回收的影响[J]. 煤炭科学技术,2024,52(5):354−363.
  • Citation
    WANG Dongdong,XIAO Li,BI Yinli,et al. Effects of AMF inoculation on plant-soil ecological stoichiometry and nutrient recovery in Shendong mine[J]. Coal Science and Technology,2024,52(5):354−363.
  • 相关文章
  • 图表

    Table1

    恢复植被土壤养分含量
    植被
    类型
    土层/cm EEG含量/(g·kg−1) TG含量/(g·kg−1) SOC含量/(g·kg−1) N含量/(g·kg−1) P含量/(g·kg−1)
    CK AM CK AM CK AM CK AM CK AM
    紫穗槐
    ZSH
    0~5 0.33±0.04BC+ 0.41±0.00b+ 1.55±0.06A*+ 1.24±0.05a*+ 3.30±0.20D*+ 6.59±0.05b*+ 0.09±0.01C* 0.16±0.00c*+ 0.18±0.01C*+ 0.29±0.04a*+
    5~10 0.20±0.02C*+ 0.29±0.03a*+ 1.26±0.04B*+ 0.87±0.04c*+ 1.47±0.03BC*+ 3.12±0.06b*+ 0.07±0.00BC* 0.13±0.00ab*+ 0.14±0.02B*+ 0.20±0.01ab*+
    沙棘
    SJ
    0~5 0.45±0.03A+ 0.53±0.02a+ 1.06±0.12C+ 0.90±0.11b 8.30±0.56A+ 9.14±0.75a+ 0.21±0.01A*+ 0.23±0.00a*+ 0.22±0.006B*+ 0.24±0.002b*+
    5~10 0.28±0.03B+ 0.22±0.00b+ 1.75±0.02A*+ 0.69±0.08d* 4.34±0.14A*+ 3.53±0.06a*+ 0.14±0.00A+ 0.14±0.01a+ 0.19±0.01A+ 0.17±0.01c+
    文冠果
    WGG
    0~5 0.38±0.01AB+ 0.37±0.00bc+ 1.02±0.12C+ 0.81±0.03b+ 2.65±0.02E+ 2.73±0.40d+ 0.09±0.00C+ 0.09±0.00e+ 0.17±0.00C+ 0.19±0.01c+
    5~10 0.28±0.02B*+ 0.14±0.02c*+ 0.74±0.02D*+ 0.50±0.05e*+ 1.10±0.16C+ 0.98±0.02d+ 0.07±0.00C+ 0.07±0.01d+ 0.14±0.01B*+ 0.16±0.00Bc*+
    欧李
    OL
    0~5 0.44±0.06A 0.44±0.05b+ 1.59±0.01A+ 1.34±0.14a 6.90±0.15B+ 6.92±0.17b+ 0.17±0.02B 0.18±0.00b+ 0.21±0.01B*+ 0.24±0.01b*+
    5~10 0.35±0.04A 0.32±0.01a+ 1.30±0.02B+ 1.17±0.08b 3.99±0.47A+ 3.26±0.19b+ 0.16±0.02A 0.12±0.00b+ 0.19±0.01A*+ 0.22±0.01a*+
    野樱桃
    YYT
    0~5 0.29±0.02C+ 0.30±0.03c+ 1.33±0.01B+ 1.25±0.08a 5.76±0.211C*+ 5.05±0.12c*+ 0.16±0.01B*+ 0.14±0.00d*+ 0.25±0.01A*+ 0.22±0.01b*
    5~10 0.21±0.00C+ 0.23±0.02b+ 0.90±0.07C*+ 1.41±0.09a* 1.92±0.071B*+ 2.28±0.11c*+ 0.09±0.00B+ 0.10±0.01c+ 0.19±0.02A+ 0.19±0.02b
      注:不同大写/小写字母代表对照区/接菌区同一土层不同植被类型的差异显著,*代表同一植被类型同一土层对照与接菌差异显著,+代表对照区或接菌区同一植被类型不同土层差异显著(P<0.05);紫穗槐,ZSH;沙棘,SJ;文冠果,WGG;欧李,OL;野樱桃,YYT;下同。

    Table2

    恢复植被各器官的养分含量
    植被类型 器官 C含量/( g·kg-1) N含量/( g·kg-1) P含量/( g·kg-1)
    CK AM CK AM CK AM
    紫穗槐
    ZSH
    405±15.33B* 442±7.24c* 13.74±0.23A* 9.82±0.01a* 3.09±0.74A 1.26±0.02b
    433±8.91C 449±12.75abc 5.90±0.38A* 7.63±0.22a* 0.85±0.06B* 2.11±0.13a*
    470±2.70B* 493±3.09a* 19.97±0.12A 20.27±0.22a 2.36±0.30A 2.82±0.17a
    466±5.74BC 471±9.54bc 8.28±0.15B 8.16±0.11a 1.56±0.00B* 0.62±0.11b*
    沙棘
    SJ
    450±15.46A 471±1.48b 5.25±0.28B 5.75±0.16b 0.50±0.05C* 2.61±0.14a*
    474±4.38AB 469±12.89ab 5.98±0.13A* 4.25±0.00b* 0.71±0.04B 0.82±0.00c
    471±3.65B* 462±2.23b* 14.01±0.28B 14.66±0.07b 1.74±0.25BC 1.95±0.00b
    493±15.02A 525±58.31a 8.36±0.24B* 7.94±0.06a* 0.55±0.06E* 0.99±0.12b*
    文冠果
    WGG
    464±1.66A* 499±1.94a* 5.08±0.27B* 4.43±0.19c* 1.21±0.01C* 0.89±0.01b*
    459±21.86BC 443±9.71bc 3.40±0.31B 4.10±0.42b 1.11±0.10A 1.41±0.15b
    512±14.26A 505±13.63a 11.90±0.47C 11.90±0.14c 2.00±0.02AB 2.14±0.30b
    487±7.84AB* 509±8.10ab* 10.30±0.45A* 6.49±1.08b* 2.76±0.09A* 1.76±0.44a*
    欧李
    OL
    446±12.80A 440±8.62c 2.65±0.31C* 1.02±0.08e* 0.76±0.13C 0.91±0.08b
    450±7.75BC 442±8.53c 2.51±0.04A* 2.96±0.06c* 0.91±0.12AB 0.81±0.00c
    466±27.01B 498±1.28a 8.24±0.15E* 8.93±0.22d* 1.24±0.11D 1.18±0.10c
    458±9.95C* 411±7.91d* 4.14±0.15D* 3.22±0.11e* 1.04±0.07D* 0.84±0.04b*
    野樱桃
    YYT
    416±19.23B 441±21.87c 2.82±0.10C 2.44±0.27d 2.32±0.15B 3.32±0.71a
    502±26.77A 470±17.86a 2.81±0.00AB 2.37±0.19d 0.65±0.21B 0.76±0.31c
    483±4.88B 451±19.49b 10.76±0.21D* 8.23±0.19e* 1.34±0.08CD 1.04±0.20c
    477±16.34ABC* 434±9.57cd* 4.94±0.00C 4.95±0.33c 1.29±0.00C* 1.55±0.18a*
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